Laboratory diagnostics: Rotavirus
What laboratory diagnostic methods can I use to diagnose rotavirus? Which one should I choose according to the situation? How do I interpret the results?
Rotavirus infections present clinically as diarrhea in nursing piglets or in the first 2 weeks after weaning
The virus is widely extended through all the swine populations of the world. Four different serotypes (A, B, C and D) affect pigs. Usually, diagnoses focus only in rotavirus serotype A (due to the difficulty to culture the rest of the serotypes). However, currently, there is a PCR test available that can classify all the serotypes. Infections in which 2 serotypes are involved are frequent. Rotavirus is present in most of the farms with almost a 100% seroconversion in the adult population. Another epidemiological feature is its persistence outside the pig, where it is resistant to environmental changes and to several disinfectants. Exposition to the virus does not necessarily mean disease. It is estimated that only 10 to 15% of diarrheas in pigs start with a primary rotavirus infection.
Symptoms
Sows
Lactating piglets
Weaners and growers
Causes / contributing factors
Diagnosis
In any diarrhea problems in pigs 10 to 14 days old, it is necessary to make sure rotavirus is the main cause through histological changes and PCR. Soak a pH indicator strip in a diarrhea sample; if the strips turns blue it is a diarrhea caused by E. coli, if the strip turns red it is a viral infection.
Control/Prevention
What laboratory diagnostic methods can I use to diagnose rotavirus? Which one should I choose according to the situation? How do I interpret the results?